Chamberlain had mixed results in eight Spring Training appearances for the Brewers. He limited opponents to three runs over eight innings for a 3.38 ERA, but he walked five batters versus two strikeouts.
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Chamberlain was one of five relief candidates released or reassigned on Wednesday, as the Brewers move closer to setting an Opening Day bullpen. Seventeen pitchers remain in the running for 12 or 13 spots.
"Generally our philosophy, especially with veteran-type players, when we come to a decision, we inform them of the decision as soon as we possibly can," Brewers general manager David Stearns said. "It's out of respect for what they've done in their careers and gives them a chance to reassess how they are going to enter this season."
"I think he pitched well," Brewers manager Craig Counsell said. "It's really we felt the other guys were ahead of him."
Chamberlain, 31, would have made $1.375 million plus incentives had he made the Brewers' Opening Day roster.
"Obviously we're working on stuff in the spring. I felt like I accomplished the things we were working on, but obviously they didn't see fit," Chamberlain said. "It's part of the game, and you understand that, wish them the best of luck and you move on from there.
"There's still time left in Spring Training. Obviously I've been healthy, I feel great, feel good with my stuff. There's a place out there somewhere, so we'll see what happens and go from there."
Chamberlain said he planned to keep tabs on the Brewers, even though he gave up his uniform on Wednesday.
"You understand there's a lot of young guys in here trying to prove some things," he said. "[I was] just trying to come in and provide leadership in a lot of ways, and you know what? I hope that in those few weeks these guys learned something in how you go about it and just to have fun and enjoy it. It doesn't last forever."
Before departing he added, "I wish them all the luck, and obviously if I latch on with a team, we'll see what happens. I hope we play them."